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KMID : 0370019960100000079
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Chung-Ang Journal of Pharmacal Sciences
1996 Volume.10 No. 0 p.79 ~ p.95
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Analysis of Oxaprozin and its Metabolites in human Urine by High Performance Liquid Chromatography and Solid Phase Extraction
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Kwon Min-Chang
Youm Jeong-Rok
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Abstract
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A new sensitive and simple method for the rapid. simultaneous pretreatment of oxaprozin and its hydroxy oxaprozin metabolites, in human urine is described. Reversed-phase High-Performance Liquid Chromatography (HPLC) employed a Cosmosil C_18 column using acetonitrile-deionized water adjusted to pH 3.25 with acetic acid as a gradient mobile phase with ultraviolet detection at 280 nm. It is essential that urine is handled rapidly and acidified upon collection prior to freezing in order to stabilize the metabolites. Urine samples first are centrifuged and oxaprozin, hydroxy oxaprozin metabolites are initially isolated by solid-phase extraction on "Waters" C_18 disposable extraction columns for sample clean-up. Upon elution, the residue is evaporated, dissolved in indomethacin (internal standard) solution and injected onto the HPLC system. One of the metabolites of oxaprozin, hydroxy oxaprozin metabolites, extracted by SPE from urine was identified by electrospray ionization mass spectrometry. The above methods are satisfactory for the analysis of oxaprozin and its metabolites in human biological fluids.
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